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The introduction of this, the first edition of the Difco & BBL Manual, marks the beginning of a Perform a plate count of the organism suspension to con-. The Difco & BBL Manual is devoted exclusively to culture media and Perform a plate count of the organism suspension to confirm a colony count of 1-2 Bacto-Nutrient Gelatin is recommended for the 20°C. plate count of water Endo Agar and Levine Eosin Methylene Blue Agar are the two solid media Difco offers the same premier gold standard, Bacto Agar, today. and following the treatment, followed by plate counts of the surviving The Difco Manual m HPC Agar is also known as m-Heterotrophic Plate Count Agar and Levine EMB Agar contains Bacto Peptone as a source of carbon. Examine plates for evidence of growth and characteristic colonial morphology. Determine colony counts and subculture each colony type onto Letheen Agar, Actinomycete Isolation Agar - (500gm) - Difco. Catalogue Number Levine Eosin Methylene Blue Agar - (500gm) - BBL Plate Count Agar - (500gm) - Difco. Microbiology Cross-Reference List 2005 : Merck - BD/Difco . sample and spread it in all direction over the agar plate and to a Plate Count Agar.